Inhibition of glycine response by amiloride in rat spinal neurons.

نویسندگان

  • Yan-Fang Li
  • Yong Li
  • Tian-Le Xu
چکیده

The modulatory effect of amiloride on glycine-activated current (I(Gly)) was investigated in acutely dissociated rat spinal dorsal horn neurons using the whole-cell patch clamp technique. Amiloride inhibited I(Gly) reversibly in a concentration-dependent manner. It shifted the concentration-response relationship to the right without altering the maximum response and Hill coefficient of the I(Gly). Amiloride did not change the ion selectivity of glycine receptor either. In addition, Na(+) - or Ca(2+) -free extracellular solutions and intracellular application of amiloride did not alter the amiloride inhibition of I(Gly). These results indicate that amiloride directly inhibited the glycine receptor response by decreasing the affinity of glycine to its receptor.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Mechanisms of H+ modulation of glycinergic response in rat sacral dorsal commissural neurons.

Many ionotropic receptors are modulated by extracellular H+. So far, few studies have directly addressed the role of such modulation at synapses. In the present study, we investigated the effects of changes in extracellular pH on glycinergic miniature inhibitory postsynaptic currents (mIPSCs) as well as glycine-evoked currents (IGly) in mechanically dissociated spinal neurons with native synapt...

متن کامل

Amiloride attenuates glycine-induced currents in cultured neurons of rat inferior colliculus.

Amiloride, a potassium sparing diuretic, is well known to interact with many ion transport systems and modulate the activity of several membrane receptors. However, relatively little information is available as to how amiloride affects membrane receptors of neurons in the brain areas. In the present study, we investigated the effects of amiloride on glycine-induced currents (I(Gly)) in cultured...

متن کامل

Amiloride inhibition of glycinergic miniature IPSCs in mechanically dissociated rat spinal neurons.

The effect of amiloride on glycinergic transmission in mechanically dissociated rat spinal dorsal horn neurons was examined with the use of whole-cell patch-clamp recording. Amiloride reversibly reduced both the frequency and amplitude of spontaneous glycinergic miniature IPSCs (mIPSCs) and its inhibitory effect on glycinergic mIPSCs persisted in either Ca2+-free or Na+-free external solutions ...

متن کامل

Role of a voltage-sensitive calcium channel blocker on inhibition of apoptosis in sensory neurons of cultured dorsal root ganglia in adult rat

Introduction: Under pathological conditions, abnormal increase in intracellular calcium concentrations is believed to induce cell death. In the present study, a voltage-sensitive calcium channel blocker (loperamide hydrochloride) was used to investigate its role in inhibition of apoptosis in sensory neurons of cultured spinal dorsal root ganglia (DRG). Methods: L5 DRG from adult rats were di...

متن کامل

Noncompetitive inhibition of the glycine receptor-mediated current by melatonin in cultured neurons.

The effect of melatonin on the glycine receptor-mediated response was studied in cultured chick spinal cord neurons using the whole-cell voltage-clamp recording technique. Melatonin rapidly and reversibly inhibited the glycine-induced current in a dose-dependent fashion, with an EC(50) of 934 microM and a maximal inhibition of 100%. Furthermore, melatonin noncompetitively inhibited the glycine ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Neuroscience letters

دوره 345 3  شماره 

صفحات  -

تاریخ انتشار 2003